Forskning ved Københavns Universitet - Københavns Universitet

Forside

Synthetic Protein Scaffolding at Biological Membranes

Publikation: Bidrag til tidsskriftReviewForskningfagfællebedømt

Protein scaffolding is a natural phenomenon whereby proteins colocalize into macromolecular complexes via specific protein–protein interactions. In the case of metabolic enzymes, protein scaffolding drives metabolic flux through specific pathways by colocalizing enzyme active sites. Synthetic protein scaffolding is increasingly used as a mechanism to improve product specificity and yields in metabolic engineering projects. To date, synthetic scaffolding has focused primarily on soluble enzyme systems, but many metabolic pathways for high-value secondary metabolites depend on membrane-bound enzymes. The compositional diversity of biological membranes and general challenges associated with modifying membrane proteins complicate scaffolding with membrane-requiring enzymes. Several recent studies have introduced new approaches to protein scaffolding at membrane surfaces, with notable success in improving product yields from specific metabolic pathways.
OriginalsprogEngelsk
TidsskriftTrends in Biotechnology
Vol/bind38
Udgave nummer4
Sider (fra-til)432-446
ISSN0167-7799
DOI
StatusUdgivet - 1 apr. 2020

ID: 230197615