Forskning ved Københavns Universitet - Københavns Universitet

Forside

The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells. / Odum, Niels; Bregenholt, S; Eriksen, K W; Skov, S; Ryder, Lars P.; Bendtzen, Klaus; Van Neerven, R J; Svejgaard, A; Garred, P.

I: HLA, Bind 54, Nr. 6, 1999, s. 572-7.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Odum, N, Bregenholt, S, Eriksen, KW, Skov, S, Ryder, LP, Bendtzen, K, Van Neerven, RJ, Svejgaard, A & Garred, P 1999, 'The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells', HLA, bind 54, nr. 6, s. 572-7. https://doi.org/10.1034/j.1399-0039.1999.540606.x

APA

Odum, N., Bregenholt, S., Eriksen, K. W., Skov, S., Ryder, L. P., Bendtzen, K., ... Garred, P. (1999). The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells. HLA, 54(6), 572-7. https://doi.org/10.1034/j.1399-0039.1999.540606.x

Vancouver

Odum N, Bregenholt S, Eriksen KW, Skov S, Ryder LP, Bendtzen K o.a. The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells. HLA. 1999;54(6):572-7. https://doi.org/10.1034/j.1399-0039.1999.540606.x

Author

Odum, Niels ; Bregenholt, S ; Eriksen, K W ; Skov, S ; Ryder, Lars P. ; Bendtzen, Klaus ; Van Neerven, R J ; Svejgaard, A ; Garred, P. / The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells. I: HLA. 1999 ; Bind 54, Nr. 6. s. 572-7.

Bibtex

@article{2ed544b0fd0711ddb219000ea68e967b,
title = "The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells",
abstract = "The CC-chemokine receptor 5 (CCR5) has recently been described as a surface marker of human T cells producing type 1 (Th1) cytokines. Here we confirm that CCR5 is expressed on human Th1 but not on Th2 T-cell clones. Using intracellular cytokine staining, we show that alloantigen specific CD4+ T-cell lines derived from a CCR5-deficient individual (delta32 allele homozygote) contain high numbers of both interferon gamma (IFN-gamma) and interleukin (IL)-2 producing cells, low numbers of IL-10 producing cells and no IL4 or IL-5 producing cells when stimulated with phorbol ester and ionomycin in vitro. These results were similar to those obtained from alloantigen specific CD4+ T-cell lines derived from CCR5 expressing individuals. An enzyme-linked immunoabsorbent assay (ELISA) confirmed that the Th1 cytokine-positive cells from the CCR5-deficient individual were able to produce equal amounts of cytokines when compared to T-cell lines from CCR5-expressing individuals, These results demonstrate that CCR5-negative T cells display the same capacity of Th1 T-cell differentiation as T cells derived from CCR5-expressing individuals. Thus, CCR5 expression is not essential for differentiation of human Th1 T cells.",
author = "Niels Odum and S Bregenholt and Eriksen, {K W} and S Skov and Ryder, {Lars P.} and Klaus Bendtzen and {Van Neerven}, {R J} and A Svejgaard and P Garred",
note = "Keywords: Biological Markers; Cell Differentiation; Cell Lineage; Cells, Cultured; Flow Cytometry; Humans; Receptors, CCR5; Th1 Cells",
year = "1999",
doi = "10.1034/j.1399-0039.1999.540606.x",
language = "English",
volume = "54",
pages = "572--7",
journal = "HLA",
issn = "2059-2302",
publisher = "Wiley",
number = "6",

}

RIS

TY - JOUR

T1 - The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells

AU - Odum, Niels

AU - Bregenholt, S

AU - Eriksen, K W

AU - Skov, S

AU - Ryder, Lars P.

AU - Bendtzen, Klaus

AU - Van Neerven, R J

AU - Svejgaard, A

AU - Garred, P

N1 - Keywords: Biological Markers; Cell Differentiation; Cell Lineage; Cells, Cultured; Flow Cytometry; Humans; Receptors, CCR5; Th1 Cells

PY - 1999

Y1 - 1999

N2 - The CC-chemokine receptor 5 (CCR5) has recently been described as a surface marker of human T cells producing type 1 (Th1) cytokines. Here we confirm that CCR5 is expressed on human Th1 but not on Th2 T-cell clones. Using intracellular cytokine staining, we show that alloantigen specific CD4+ T-cell lines derived from a CCR5-deficient individual (delta32 allele homozygote) contain high numbers of both interferon gamma (IFN-gamma) and interleukin (IL)-2 producing cells, low numbers of IL-10 producing cells and no IL4 or IL-5 producing cells when stimulated with phorbol ester and ionomycin in vitro. These results were similar to those obtained from alloantigen specific CD4+ T-cell lines derived from CCR5 expressing individuals. An enzyme-linked immunoabsorbent assay (ELISA) confirmed that the Th1 cytokine-positive cells from the CCR5-deficient individual were able to produce equal amounts of cytokines when compared to T-cell lines from CCR5-expressing individuals, These results demonstrate that CCR5-negative T cells display the same capacity of Th1 T-cell differentiation as T cells derived from CCR5-expressing individuals. Thus, CCR5 expression is not essential for differentiation of human Th1 T cells.

AB - The CC-chemokine receptor 5 (CCR5) has recently been described as a surface marker of human T cells producing type 1 (Th1) cytokines. Here we confirm that CCR5 is expressed on human Th1 but not on Th2 T-cell clones. Using intracellular cytokine staining, we show that alloantigen specific CD4+ T-cell lines derived from a CCR5-deficient individual (delta32 allele homozygote) contain high numbers of both interferon gamma (IFN-gamma) and interleukin (IL)-2 producing cells, low numbers of IL-10 producing cells and no IL4 or IL-5 producing cells when stimulated with phorbol ester and ionomycin in vitro. These results were similar to those obtained from alloantigen specific CD4+ T-cell lines derived from CCR5 expressing individuals. An enzyme-linked immunoabsorbent assay (ELISA) confirmed that the Th1 cytokine-positive cells from the CCR5-deficient individual were able to produce equal amounts of cytokines when compared to T-cell lines from CCR5-expressing individuals, These results demonstrate that CCR5-negative T cells display the same capacity of Th1 T-cell differentiation as T cells derived from CCR5-expressing individuals. Thus, CCR5 expression is not essential for differentiation of human Th1 T cells.

U2 - 10.1034/j.1399-0039.1999.540606.x

DO - 10.1034/j.1399-0039.1999.540606.x

M3 - Journal article

C2 - 10674971

VL - 54

SP - 572

EP - 577

JO - HLA

JF - HLA

SN - 2059-2302

IS - 6

ER -

ID: 10617426