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The cis/trans interconversion of the calcium regulating hormone calcitonin is catalyzed by cyclophilin

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • D Kern
  • T Drakenberg
  • M Wikström
  • S Forsén
  • H Bang
  • G Fischer
The cytosolic peptidyl-prolyl cis/trans isomerase cyclophilin from pig kidney can accelerate catalytically the cis/trans isomerization of prolyl peptide bonds. One- and two-dimensional 1H NMR spectroscopy was used to prove that the polypeptide hormone calcitonin is a substrate for cyclophilin. Isomerization of only one of the two prolyl peptide bonds is catalyzed significantly. The efficiency of catalysis was calculated by lineshape analysis and NOESY spectroscopy. Cyclosporin A completely blocks the effect of the enzyme on the conformational dynamics of the polypeptide.
OriginalsprogEngelsk
TidsskriftFEBS letters
Vol/bind323
Udgave nummer3
Sider (fra-til)198-202
Antal sider5
ISSN0014-5793
DOI
StatusUdgivet - 1 jun. 1993
Eksternt udgivetJa

ID: 50166461