Forskning ved Københavns Universitet - Københavns Universitet


The Dissolution of Double Holliday Junctions

Publikation: Bidrag til tidsskriftReviewForskningfagfællebedømt

Double Holliday junctions (dHJS) are important intermediates of homologous recombination. The separate junctions can each be cleaved by DNA structure-selective endonucleases known as Holliday junction resolvases. Alternatively, double Holliday junctions can be processed by a reaction known as "double Holliday junction dissolution." This reaction requires the cooperative action of a so-called "dissolvasome" comprising a Holliday junction branch migration enzyme (Sgs1/BLM RecQ helicase) and a type IA topoisomerase (Top3/TopoIIIα) in complex with its OB (oligonucleotide/oligosaccharide binding) fold containing accessory factor (Rmi1). This review details our current knowledge of the dissolution process and the players involved in catalyzing this mechanistically complex means of completing homologous recombination reactions.

TidsskriftCold Spring Harbor perspectives in biology
Sider (fra-til)1-16
Antal sider16
StatusUdgivet - 1 jul. 2014

ID: 119170260