Forskning ved Københavns Universitet - Københavns Universitet

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The effect of oxygen tension on porcine embryonic development is dependent on embryo type.

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Reducing oxygen concentration from atmospheric levels during in vitro culture generally, but not invariably, improves embryonic development across a range of species. Since the few published reports of such an action in the pig are contradictory--perhaps a consequence of the derivation of the embryos prior to culture--a study was performed to examine the effect of O2 tension during culture on three different types of porcine embryos, namely: in vivo flushed embryos, and in vitro matured oocytes either fertilized in vitro or parthenogenetically activated. In vivo embryos (n=208) were flushed at the 2-8 cell stage. Cumulus oocyte complexes (COCs) destined for IVF or parthenogenetic activation were derived from 2 to 6 mm, post-pubertal ovarian follicles and matured for 48 h in TCM-199. Parthenogenones were generated by activating denuded oocytes (n=573) with 10 mM calcium ionophore, followed by 2 mM DMAP prior to culture. The IVF embryos (n=971) were produced by fertilizing COCs (day 0) with fresh ejaculated semen in modified tris-based medium for 6 h before cumulus removal. All embryos were cultured in BECM-3 containing 12 mg/mL fatty-acid-free BSA up to day 4, followed by BECM-3 supplemented with 10% calf serum until day 7. The gas environment for IVM/IVF was 5% CO2 in air, while that for IVC was either 5% CO2 in air or 5% O2, 5% CO2 and 90% N2. Low O2 tension increased both day 7 blastocyst rates (high versus low O2, respectively; 9.3+/-2.9%: 26/280; 23.9+/-4.2%: 71/293; P
OriginalsprogEngelsk
TidsskriftTheriogenology
Vol/bind63
Udgave nummer7
Sider (fra-til)2040-2052
Antal sider13
ISSN0093-691X
DOI
StatusUdgivet - apr. 2005

    Forskningsområder

  • Animals, Blastocyst, Blastocyst: physiology, Culture Techniques, Culture Techniques: methods, Culture Techniques: veterinary, Embryonic Development, Embryonic Development: physiology, Female, Fertilization in Vitro, Fertilization in Vitro: methods, Fertilization in Vitro: veterinary, Insemination, Artificial, Insemination, Artificial: veterinary, Logistic Models, Male, Oxygen, Oxygen: administration & dosage, Parthenogenesis, Parthenogenesis: physiology, Pregnancy, Swine, Swine: embryology

ID: 141543436