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The effect of oxygen tension on porcine embryonic development is dependent on embryo type.

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The effect of oxygen tension on porcine embryonic development is dependent on embryo type. / Booth, Paul J; Holm, Peter; Callesen, Henrik.

I: Theriogenology, Bind 63, Nr. 7, 04.2005, s. 2040-2052.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Booth, PJ, Holm, P & Callesen, H 2005, 'The effect of oxygen tension on porcine embryonic development is dependent on embryo type.', Theriogenology, bind 63, nr. 7, s. 2040-2052. https://doi.org/10.1016/j.theriogenology.2004.10.001

APA

Booth, P. J., Holm, P., & Callesen, H. (2005). The effect of oxygen tension on porcine embryonic development is dependent on embryo type. Theriogenology, 63(7), 2040-2052. https://doi.org/10.1016/j.theriogenology.2004.10.001

Vancouver

Booth PJ, Holm P, Callesen H. The effect of oxygen tension on porcine embryonic development is dependent on embryo type. Theriogenology. 2005 apr;63(7):2040-2052. https://doi.org/10.1016/j.theriogenology.2004.10.001

Author

Booth, Paul J ; Holm, Peter ; Callesen, Henrik. / The effect of oxygen tension on porcine embryonic development is dependent on embryo type. I: Theriogenology. 2005 ; Bind 63, Nr. 7. s. 2040-2052.

Bibtex

@article{283179ea4b6044b297caf19e2f2c27ff,
title = "The effect of oxygen tension on porcine embryonic development is dependent on embryo type.",
abstract = "Reducing oxygen concentration from atmospheric levels during in vitro culture generally, but not invariably, improves embryonic development across a range of species. Since the few published reports of such an action in the pig are contradictory--perhaps a consequence of the derivation of the embryos prior to culture--a study was performed to examine the effect of O2 tension during culture on three different types of porcine embryos, namely: in vivo flushed embryos, and in vitro matured oocytes either fertilized in vitro or parthenogenetically activated. In vivo embryos (n=208) were flushed at the 2-8 cell stage. Cumulus oocyte complexes (COCs) destined for IVF or parthenogenetic activation were derived from 2 to 6 mm, post-pubertal ovarian follicles and matured for 48 h in TCM-199. Parthenogenones were generated by activating denuded oocytes (n=573) with 10 mM calcium ionophore, followed by 2 mM DMAP prior to culture. The IVF embryos (n=971) were produced by fertilizing COCs (day 0) with fresh ejaculated semen in modified tris-based medium for 6 h before cumulus removal. All embryos were cultured in BECM-3 containing 12 mg/mL fatty-acid-free BSA up to day 4, followed by BECM-3 supplemented with 10{\%} calf serum until day 7. The gas environment for IVM/IVF was 5{\%} CO2 in air, while that for IVC was either 5{\%} CO2 in air or 5{\%} O2, 5{\%} CO2 and 90{\%} N2. Low O2 tension increased both day 7 blastocyst rates (high versus low O2, respectively; 9.3+/-2.9{\%}: 26/280; 23.9+/-4.2{\%}: 71/293; P",
keywords = "Animals, Blastocyst, Blastocyst: physiology, Culture Techniques, Culture Techniques: methods, Culture Techniques: veterinary, Embryonic Development, Embryonic Development: physiology, Female, Fertilization in Vitro, Fertilization in Vitro: methods, Fertilization in Vitro: veterinary, Insemination, Artificial, Insemination, Artificial: veterinary, Logistic Models, Male, Oxygen, Oxygen: administration & dosage, Parthenogenesis, Parthenogenesis: physiology, Pregnancy, Swine, Swine: embryology",
author = "Booth, {Paul J} and Peter Holm and Henrik Callesen",
year = "2005",
month = "4",
doi = "10.1016/j.theriogenology.2004.10.001",
language = "English",
volume = "63",
pages = "2040--2052",
journal = "Theriogenology",
issn = "0093-691X",
publisher = "Elsevier",
number = "7",

}

RIS

TY - JOUR

T1 - The effect of oxygen tension on porcine embryonic development is dependent on embryo type.

AU - Booth, Paul J

AU - Holm, Peter

AU - Callesen, Henrik

PY - 2005/4

Y1 - 2005/4

N2 - Reducing oxygen concentration from atmospheric levels during in vitro culture generally, but not invariably, improves embryonic development across a range of species. Since the few published reports of such an action in the pig are contradictory--perhaps a consequence of the derivation of the embryos prior to culture--a study was performed to examine the effect of O2 tension during culture on three different types of porcine embryos, namely: in vivo flushed embryos, and in vitro matured oocytes either fertilized in vitro or parthenogenetically activated. In vivo embryos (n=208) were flushed at the 2-8 cell stage. Cumulus oocyte complexes (COCs) destined for IVF or parthenogenetic activation were derived from 2 to 6 mm, post-pubertal ovarian follicles and matured for 48 h in TCM-199. Parthenogenones were generated by activating denuded oocytes (n=573) with 10 mM calcium ionophore, followed by 2 mM DMAP prior to culture. The IVF embryos (n=971) were produced by fertilizing COCs (day 0) with fresh ejaculated semen in modified tris-based medium for 6 h before cumulus removal. All embryos were cultured in BECM-3 containing 12 mg/mL fatty-acid-free BSA up to day 4, followed by BECM-3 supplemented with 10% calf serum until day 7. The gas environment for IVM/IVF was 5% CO2 in air, while that for IVC was either 5% CO2 in air or 5% O2, 5% CO2 and 90% N2. Low O2 tension increased both day 7 blastocyst rates (high versus low O2, respectively; 9.3+/-2.9%: 26/280; 23.9+/-4.2%: 71/293; P

AB - Reducing oxygen concentration from atmospheric levels during in vitro culture generally, but not invariably, improves embryonic development across a range of species. Since the few published reports of such an action in the pig are contradictory--perhaps a consequence of the derivation of the embryos prior to culture--a study was performed to examine the effect of O2 tension during culture on three different types of porcine embryos, namely: in vivo flushed embryos, and in vitro matured oocytes either fertilized in vitro or parthenogenetically activated. In vivo embryos (n=208) were flushed at the 2-8 cell stage. Cumulus oocyte complexes (COCs) destined for IVF or parthenogenetic activation were derived from 2 to 6 mm, post-pubertal ovarian follicles and matured for 48 h in TCM-199. Parthenogenones were generated by activating denuded oocytes (n=573) with 10 mM calcium ionophore, followed by 2 mM DMAP prior to culture. The IVF embryos (n=971) were produced by fertilizing COCs (day 0) with fresh ejaculated semen in modified tris-based medium for 6 h before cumulus removal. All embryos were cultured in BECM-3 containing 12 mg/mL fatty-acid-free BSA up to day 4, followed by BECM-3 supplemented with 10% calf serum until day 7. The gas environment for IVM/IVF was 5% CO2 in air, while that for IVC was either 5% CO2 in air or 5% O2, 5% CO2 and 90% N2. Low O2 tension increased both day 7 blastocyst rates (high versus low O2, respectively; 9.3+/-2.9%: 26/280; 23.9+/-4.2%: 71/293; P

KW - Animals

KW - Blastocyst

KW - Blastocyst: physiology

KW - Culture Techniques

KW - Culture Techniques: methods

KW - Culture Techniques: veterinary

KW - Embryonic Development

KW - Embryonic Development: physiology

KW - Female

KW - Fertilization in Vitro

KW - Fertilization in Vitro: methods

KW - Fertilization in Vitro: veterinary

KW - Insemination, Artificial

KW - Insemination, Artificial: veterinary

KW - Logistic Models

KW - Male

KW - Oxygen

KW - Oxygen: administration & dosage

KW - Parthenogenesis

KW - Parthenogenesis: physiology

KW - Pregnancy

KW - Swine

KW - Swine: embryology

U2 - 10.1016/j.theriogenology.2004.10.001

DO - 10.1016/j.theriogenology.2004.10.001

M3 - Journal article

C2 - 15823359

VL - 63

SP - 2040

EP - 2052

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

IS - 7

ER -

ID: 141543436