Forskning ved Københavns Universitet - Københavns Universitet


The effects of grain-induced subactue ruminal acidosis on interleukin-6 and acute phase response in dairy cows

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskningfagfællebedømt

  • Shucong Li
  • Anne Mette Danscher
  • Pia Haubro Andersen
  • Ehsan Khafipour
  • Niels Bastian Kristensen
  • Jan C. Plaizier
Subacute ruminal acidosis (SARA) resulting from excessive grain feeding to dairy cows is accompanied by an
acute phase response. Interleukin 6 (IL-6) has been proposed as a mediator of this response. We tested if the
acute phase response associated with grain-induced SARA is mediated by IL-6. Six lactating Danish Holstein
cows were used in a cross over study that included two periods with two cows on a control diet and two cows on
a SARA-challenge diet, and a third period with two cows on the SARA-challenge diet. Control cows received a
total mixed ration (174 g crude protein/kg DM; 192 g starch/kg DM; 6.28 MJ NEL/kg DM) for two weeks before
40% this diet was gradually substituted with grain pellets (50:50 wheat:barley) within 3 days. The SARA diet was
fed for another four days before switching back to the control diet. Blood samples were obtained from the jugular
vein at 7 h post-feeding on d 5, and d 3 before SARA induction and during three SARA days. ELISA kits were
used for measurement of IL-6, and the acute phase proteins haptoglobin (Hp), LPS binding protein (LBP), and
serum amyloid A (SAA). The SARA challenge tended to increase LBP (7.54 vs. 10.23 mg/L, P = 0.10), and
increased the SAA (4.24 vs. 11.60 mg/L, P = 0.04) and Hp (3.57 vs. 22.09 mg/L, P = 0.04) in blood plasma,
confirming that the challenge caused an acute phase response. Concentrations of IL-6 were not affected by the
SARA challenge, and averaged 5.06 ng/mL across treatments. Our data do not confirm that IL-6 mediates the
acute phase response during grain-induced SARA. This conformation may require more frequent blood sampling
around the time of the SARA challenge.
TidsskriftJournal of Animal Science
Udgave nummerE suppl. 2
Sider (fra-til)49
Antal sider1
StatusUdgivet - 20 jul. 2014
Begivenhed2014 JAM joint annual meeting: Linking animal science and animal agriculture: - Kanasas City, USA
Varighed: 20 jul. 201424 jul. 2014


Konference2014 JAM joint annual meeting
ByKanasas City

ID: 140440524