Forskning ved Københavns Universitet - Københavns Universitet

Forside

The membrane fraction of homogenized rat kidney contains an enzyme that releases epidermal growth factor from the kidney membranes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

The membrane fraction of homogenized rat kidney contains an enzyme that releases epidermal growth factor from the kidney membranes. / Nexø, Ebba; Poulsen, Steen Seier.

I: BBA General Subjects, Bind 1074, Nr. 2, 08.07.1991, s. 284-88.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Nexø, E & Poulsen, SS 1991, 'The membrane fraction of homogenized rat kidney contains an enzyme that releases epidermal growth factor from the kidney membranes', BBA General Subjects, bind 1074, nr. 2, s. 284-88.

APA

Nexø, E., & Poulsen, S. S. (1991). The membrane fraction of homogenized rat kidney contains an enzyme that releases epidermal growth factor from the kidney membranes. BBA General Subjects, 1074(2), 284-88.

Vancouver

Nexø E, Poulsen SS. The membrane fraction of homogenized rat kidney contains an enzyme that releases epidermal growth factor from the kidney membranes. BBA General Subjects. 1991 jul 8;1074(2):284-88.

Author

Nexø, Ebba ; Poulsen, Steen Seier. / The membrane fraction of homogenized rat kidney contains an enzyme that releases epidermal growth factor from the kidney membranes. I: BBA General Subjects. 1991 ; Bind 1074, Nr. 2. s. 284-88.

Bibtex

@article{58c55ab074d011dbbee902004c4f4f50,
title = "The membrane fraction of homogenized rat kidney contains an enzyme that releases epidermal growth factor from the kidney membranes",
abstract = "High levels of epidermal growth factor (EGF) are excreted in the urine and high levels of mRNA for the EGF-precursor have been demonstrated in the kidney. The EGF-precursor is a membrane bound peptide in the kidney, but little is known about the renal processing of the precursor. The present study shows that the membrane fraction of homogenized rat kidney contains an enzyme that releases immuno and receptor reactive EGF from the kidney membranes when incubated at 37 degrees C. Gel filtration shows that the EGF reactivity released from the membranes is similar to the EGF reactivity in rat urine. The EGF releasing enzyme is inhibited by the serine proteinase inhibitor aprotinin and by low temperatures (4 degrees C). The pH optimum of the reaction is pH 7.5-8.0.",
keywords = "Animals, Chromatography, Gel, Enzyme-Linked Immunosorbent Assay, Epidermal Growth Factor, Hydrogen-Ion Concentration, Kidney, Male, Radioligand Assay, Rats, Rats, Inbred Strains, Subcellular Fractions, Temperature",
author = "Ebba Nex{\o} and Poulsen, {Steen Seier}",
year = "1991",
month = jul,
day = "8",
language = "English",
volume = "1074",
pages = "284--88",
journal = "B B A - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - The membrane fraction of homogenized rat kidney contains an enzyme that releases epidermal growth factor from the kidney membranes

AU - Nexø, Ebba

AU - Poulsen, Steen Seier

PY - 1991/7/8

Y1 - 1991/7/8

N2 - High levels of epidermal growth factor (EGF) are excreted in the urine and high levels of mRNA for the EGF-precursor have been demonstrated in the kidney. The EGF-precursor is a membrane bound peptide in the kidney, but little is known about the renal processing of the precursor. The present study shows that the membrane fraction of homogenized rat kidney contains an enzyme that releases immuno and receptor reactive EGF from the kidney membranes when incubated at 37 degrees C. Gel filtration shows that the EGF reactivity released from the membranes is similar to the EGF reactivity in rat urine. The EGF releasing enzyme is inhibited by the serine proteinase inhibitor aprotinin and by low temperatures (4 degrees C). The pH optimum of the reaction is pH 7.5-8.0.

AB - High levels of epidermal growth factor (EGF) are excreted in the urine and high levels of mRNA for the EGF-precursor have been demonstrated in the kidney. The EGF-precursor is a membrane bound peptide in the kidney, but little is known about the renal processing of the precursor. The present study shows that the membrane fraction of homogenized rat kidney contains an enzyme that releases immuno and receptor reactive EGF from the kidney membranes when incubated at 37 degrees C. Gel filtration shows that the EGF reactivity released from the membranes is similar to the EGF reactivity in rat urine. The EGF releasing enzyme is inhibited by the serine proteinase inhibitor aprotinin and by low temperatures (4 degrees C). The pH optimum of the reaction is pH 7.5-8.0.

KW - Animals

KW - Chromatography, Gel

KW - Enzyme-Linked Immunosorbent Assay

KW - Epidermal Growth Factor

KW - Hydrogen-Ion Concentration

KW - Kidney

KW - Male

KW - Radioligand Assay

KW - Rats

KW - Rats, Inbred Strains

KW - Subcellular Fractions

KW - Temperature

M3 - Journal article

C2 - 2065081

VL - 1074

SP - 284

EP - 288

JO - B B A - General Subjects

JF - B B A - General Subjects

SN - 0304-4165

IS - 2

ER -

ID: 293336