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The potential role of SOCS-3 in the interleukin-1beta-induced desensitization of insulin signaling in pancreatic beta-cells

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Emanuelli, Brice
  • Murielle Glondu
  • Chantal Filloux
  • Pascal Peraldi
  • Emmanuel Van Obberghen

Defects in insulin secretion, resulting from loss of function or destruction of pancreatic beta-cells, trigger diabetes. Interleukin (IL)-1beta is a proinflammatory cytokine that is involved in type 1 and type 2 diabetes development and impairs beta-cell survival and function. Because effective insulin signaling is required for the optimal beta-cell function, we assessed the effect of IL-1beta on the insulin pathway in a rat pancreatic beta-cell line. We show that IL-1beta decreases insulin-induced tyrosine phosphorylation of the insulin receptor (IR) and insulin receptor substrate (IRS) proteins as well as phosphatidylinositol 3-kinase (PI3K) activation, and that this action is not due to the IL-1beta-dependent nitric oxide (NO) production in RINm5F cells. We next analyzed if suppressor of cytokine signaling (SOCS)-3, which can be induced by multiple cytokines and which we identified as an insulin action inhibitor, was implicated in the IL-1beta inhibitory effect on insulin signaling in these cells. We show that IL-1beta increases SOCS-3 expression and induces SOCS-3/IR complex formation in RINm5F cells. Moreover, we find that ectopically expressed SOCS-3 associates with the IR and reduces insulin-dependent IR autophosphorylation and IRS/PI3K pathway in a way comparable to IL-1beta treatment in RINm5F cells. We propose that IL-1beta decreases insulin action in beta-cells through the induction of SOCS-3 expression, and that this effect potentially alters insulin-induced beta-cell survival.

OriginalsprogEngelsk
TidsskriftDiabetes
Vol/bind53 Suppl 3
Sider (fra-til)S97-S103
ISSN0012-1797
StatusUdgivet - dec. 2004

    Forskningsområder

  • Animals, Cell Line, Tumor, Cloning, Molecular, DNA, Complementary, Gene Expression Regulation, Neoplastic, Insulin, Insulinoma, Interleukin-1, Islets of Langerhans, Pancreatic Neoplasms, Phosphorylation, RNA, Messenger, Rats, Recombinant Proteins, Repressor Proteins, Signal Transduction, Suppressor of Cytokine Signaling Proteins, Transcription Factors

ID: 143328742