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The presence of inositol phosphates in gastric pig digesta is affected by time after feeding a nonfermented or fermented liquid wheat- barley-based diet

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The objective was to quantify the retention of digesta and evaluate the degradation of phytate or inositol hexakisphosphate (InsP(6)) and lower inositol phosphates (InsP(5), InsP(4), InsP(3), and InsP(2)) in the stomach at different times after feeding pigs a fermented liquid diet with microbial phytase or a nonfermented diet with or without microbial phytase. Six barrows fitted with gastric cannulas were used. The experiment was a 3 × 3 Latin square with 3 pigs fed 3 diets during 3 wk in 2 replicates. Each experimental period lasted for 7 d, comprising 3 d of adaptation and 4 d of total collection of gastric digesta. For each pig, the digesta was collected once daily at 1, 2, 3, or 5 h after feeding the morning meal. A basal wheat- and barley-based diet was steam-pelleted at 90°C. The dietary treatments were a nonfermented basal diet (NF-BD), the NF-BD with microbial phytase (750 phytase units of phytase/kg, as-fed basis; NF-BD + phytase), and the NF-BD + phytase fermented for 17.5 h (F-BD + phytase). Gastric InsP(6)-P was not detected at all in pigs fed F-BD + phytase because of complete InsP(6) degradation during fermentation of the feed before feeding. Gastric InsP(6)-P decreased over time (P < 0.05) in pigs fed NF-BD and NF-BD + phytase. The decreases were 45, 54, 56, and 61 percentage points greater at 1, 2, 3, and 5 h, respectively, in pigs fed NF-BD + phytase compared with NF-BD. However, substantial amounts of InsP(6) still passed into the small intestine in pigs fed NF-BD + phytase, especially within the first hour (estimated to 17% of InsP(6)-P intake). The accumulation of lower inositol phosphates in gastric digesta was very small for all treatments and at all times because of a rapid and almost complete degradation. In conclusion, phytase addition to the nonfermented diet increased the degradation of gastric InsP(6). However, considerable amounts of intact InsP(6) still passed into the small intestine because of a shortage of time for InsP(6) degradation in the stomach. Therefore, to increase the apparent digestibility of plant P in dry wheat- and barley-based diets, the development of phytases that can degrade InsP(6) effectively immediately after ingestion of the feed at an initial gastric pH from 6.5 to 5.0 is needed. Feeding F-BD + phytase compensated for the shortage of time because the InsP(6) degradation was completed during fermentation before feeding. The degradation of InsP(6) to InsP(5) is the bottleneck for plant P utilization in pigs because the degradation of the lower inositol phosphates is rapid and almost complete.
OriginalsprogEngelsk
TidsskriftJournal of Animal Science
Vol/bind89
Udgave nummer10
Sider (fra-til)3153-3162
Antal sider10
ISSN0021-8812
DOI
StatusUdgivet - 2011

ID: 35082779