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The role of dam methyltransferase in the control of DNA replication in E. coli

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Standard

The role of dam methyltransferase in the control of DNA replication in E. coli. / Boye, E; Løbner-Olesen, A.

I: Cell, Bind 62, Nr. 5, 07.09.1990, s. 981-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Boye, E & Løbner-Olesen, A 1990, 'The role of dam methyltransferase in the control of DNA replication in E. coli', Cell, bind 62, nr. 5, s. 981-9.

APA

Boye, E., & Løbner-Olesen, A. (1990). The role of dam methyltransferase in the control of DNA replication in E. coli. Cell, 62(5), 981-9.

Vancouver

Boye E, Løbner-Olesen A. The role of dam methyltransferase in the control of DNA replication in E. coli. Cell. 1990 sep 7;62(5):981-9.

Author

Boye, E ; Løbner-Olesen, A. / The role of dam methyltransferase in the control of DNA replication in E. coli. I: Cell. 1990 ; Bind 62, Nr. 5. s. 981-9.

Bibtex

@article{dab4782be8e64ba6825ebde6fdac20e9,
title = "The role of dam methyltransferase in the control of DNA replication in E. coli",
abstract = "The timing and control of initiation of DNA replication in E. coli was studied under conditions where the cellular level of dam methyltransferase was controlled by a temperature-inducible promoter. Flow cytometry was used to demonstrate that the synchrony of initiation at the several origins within each cell was critically dependent on the level of dam methyltransferase. Initiations were shown to be synchronous only in a narrow temperature range. The data are explained by a model where a newly replicated and therefore hemimethylated oriC is inert for reinitiation. Such a model may be applicable to eukaryotic cells, where classes of origins are initiated in synchrony and only once per cell cycle.",
keywords = "DNA Replication, DNA, Bacterial/biosynthesis, Escherichia coli/enzymology, Escherichia coli Proteins, Kinetics, Methyltransferases/genetics, Phenotype, Promoter Regions, Genetic, SOS Response (Genetics), Site-Specific DNA-Methyltransferase (Adenine-Specific), Temperature",
author = "E Boye and A L{\o}bner-Olesen",
year = "1990",
month = "9",
day = "7",
language = "English",
volume = "62",
pages = "981--9",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - The role of dam methyltransferase in the control of DNA replication in E. coli

AU - Boye, E

AU - Løbner-Olesen, A

PY - 1990/9/7

Y1 - 1990/9/7

N2 - The timing and control of initiation of DNA replication in E. coli was studied under conditions where the cellular level of dam methyltransferase was controlled by a temperature-inducible promoter. Flow cytometry was used to demonstrate that the synchrony of initiation at the several origins within each cell was critically dependent on the level of dam methyltransferase. Initiations were shown to be synchronous only in a narrow temperature range. The data are explained by a model where a newly replicated and therefore hemimethylated oriC is inert for reinitiation. Such a model may be applicable to eukaryotic cells, where classes of origins are initiated in synchrony and only once per cell cycle.

AB - The timing and control of initiation of DNA replication in E. coli was studied under conditions where the cellular level of dam methyltransferase was controlled by a temperature-inducible promoter. Flow cytometry was used to demonstrate that the synchrony of initiation at the several origins within each cell was critically dependent on the level of dam methyltransferase. Initiations were shown to be synchronous only in a narrow temperature range. The data are explained by a model where a newly replicated and therefore hemimethylated oriC is inert for reinitiation. Such a model may be applicable to eukaryotic cells, where classes of origins are initiated in synchrony and only once per cell cycle.

KW - DNA Replication

KW - DNA, Bacterial/biosynthesis

KW - Escherichia coli/enzymology

KW - Escherichia coli Proteins

KW - Kinetics

KW - Methyltransferases/genetics

KW - Phenotype

KW - Promoter Regions, Genetic

KW - SOS Response (Genetics)

KW - Site-Specific DNA-Methyltransferase (Adenine-Specific)

KW - Temperature

M3 - Journal article

C2 - 2203541

VL - 62

SP - 981

EP - 989

JO - Cell

JF - Cell

SN - 0092-8674

IS - 5

ER -

ID: 200973288