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The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor

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In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.
OriginalsprogEngelsk
TidsskriftMolecular and General Genetics MGG
Vol/bind255
Udgave nummer2
Sider (fra-til)226-36
Antal sider11
ISSN0026-8925
StatusUdgivet - 1 jun. 1997

ID: 33576971