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Transcriptional Silencing of Retroviral Vectors.

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Standard

Transcriptional Silencing of Retroviral Vectors. / Lund, Anders Henrik; Duch, M.; Pedersen, F.S.

I: Journal of Biomedical Science, Bind 3, Nr. 6, 1996, s. 365-378.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Lund, AH, Duch, M & Pedersen, FS 1996, 'Transcriptional Silencing of Retroviral Vectors.', Journal of Biomedical Science, bind 3, nr. 6, s. 365-378.

APA

Lund, A. H., Duch, M., & Pedersen, F. S. (1996). Transcriptional Silencing of Retroviral Vectors. Journal of Biomedical Science, 3(6), 365-378.

Vancouver

Lund AH, Duch M, Pedersen FS. Transcriptional Silencing of Retroviral Vectors. Journal of Biomedical Science. 1996;3(6):365-378.

Author

Lund, Anders Henrik ; Duch, M. ; Pedersen, F.S. / Transcriptional Silencing of Retroviral Vectors. I: Journal of Biomedical Science. 1996 ; Bind 3, Nr. 6. s. 365-378.

Bibtex

@article{7e615b60526f11dd8d9f000ea68e967b,
title = "Transcriptional Silencing of Retroviral Vectors.",
abstract = "Although retroviral vector systems have been found to efficiently transduce a variety of cell types in vitro, the use of vectors based on murine leukemia virus in preclinical models of somatic gene therapy has led to the identification of transcriptional silencing in vivo as an important problem. Extinction of long-term vector expression has been observed after implantation of transduced hematopoietic cells as well as fibroblasts, myoblasts and hepatocytes. Here we review the influence of vector structure, integration site and cell type on transcriptional silencing. While down-regulation of proviral transcription is known from a number of cellular and animal models, major insight has been gained from studies in the germ line and embryonal cells of the mouse. Key elements for the transfer and expression of retroviral vectors, such as the viral transcriptional enhancer and the binding site for the tRNA primer for reverse transcription may have a major influence on transcriptional silencing. Alterations of these elements of the vector backbone as well as the use of internal promoter elements from housekeeping genes may contribute to reduce transcriptional silencing. The use of cell culture and animal models in the testing and improvement of vector design is discussed. Copyright 1996 S. Karger AG, Basel",
author = "Lund, {Anders Henrik} and M. Duch and F.S. Pedersen",
year = "1996",
language = "English",
volume = "3",
pages = "365--378",
journal = "Journal of Biomedical Science",
issn = "1021-7770",
publisher = "BioMed Central Ltd.",
number = "6",

}

RIS

TY - JOUR

T1 - Transcriptional Silencing of Retroviral Vectors.

AU - Lund, Anders Henrik

AU - Duch, M.

AU - Pedersen, F.S.

PY - 1996

Y1 - 1996

N2 - Although retroviral vector systems have been found to efficiently transduce a variety of cell types in vitro, the use of vectors based on murine leukemia virus in preclinical models of somatic gene therapy has led to the identification of transcriptional silencing in vivo as an important problem. Extinction of long-term vector expression has been observed after implantation of transduced hematopoietic cells as well as fibroblasts, myoblasts and hepatocytes. Here we review the influence of vector structure, integration site and cell type on transcriptional silencing. While down-regulation of proviral transcription is known from a number of cellular and animal models, major insight has been gained from studies in the germ line and embryonal cells of the mouse. Key elements for the transfer and expression of retroviral vectors, such as the viral transcriptional enhancer and the binding site for the tRNA primer for reverse transcription may have a major influence on transcriptional silencing. Alterations of these elements of the vector backbone as well as the use of internal promoter elements from housekeeping genes may contribute to reduce transcriptional silencing. The use of cell culture and animal models in the testing and improvement of vector design is discussed. Copyright 1996 S. Karger AG, Basel

AB - Although retroviral vector systems have been found to efficiently transduce a variety of cell types in vitro, the use of vectors based on murine leukemia virus in preclinical models of somatic gene therapy has led to the identification of transcriptional silencing in vivo as an important problem. Extinction of long-term vector expression has been observed after implantation of transduced hematopoietic cells as well as fibroblasts, myoblasts and hepatocytes. Here we review the influence of vector structure, integration site and cell type on transcriptional silencing. While down-regulation of proviral transcription is known from a number of cellular and animal models, major insight has been gained from studies in the germ line and embryonal cells of the mouse. Key elements for the transfer and expression of retroviral vectors, such as the viral transcriptional enhancer and the binding site for the tRNA primer for reverse transcription may have a major influence on transcriptional silencing. Alterations of these elements of the vector backbone as well as the use of internal promoter elements from housekeeping genes may contribute to reduce transcriptional silencing. The use of cell culture and animal models in the testing and improvement of vector design is discussed. Copyright 1996 S. Karger AG, Basel

M3 - Journal article

C2 - 11725119

VL - 3

SP - 365

EP - 378

JO - Journal of Biomedical Science

JF - Journal of Biomedical Science

SN - 1021-7770

IS - 6

ER -

ID: 5016642