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Ultrastructure of the endolymphatic duct in the rat. Fixation and preservation

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Ultrastructure of the endolymphatic duct in the rat. Fixation and preservation. / Qvortrup, K; Rostgaard, J.

I: Acta Oto-Laryngologica. Supplement, Bind 113, Nr. 6, 11.1993, s. 731-40.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Qvortrup, K & Rostgaard, J 1993, 'Ultrastructure of the endolymphatic duct in the rat. Fixation and preservation', Acta Oto-Laryngologica. Supplement, bind 113, nr. 6, s. 731-40.

APA

Qvortrup, K., & Rostgaard, J. (1993). Ultrastructure of the endolymphatic duct in the rat. Fixation and preservation. Acta Oto-Laryngologica. Supplement, 113(6), 731-40.

Vancouver

Qvortrup K, Rostgaard J. Ultrastructure of the endolymphatic duct in the rat. Fixation and preservation. Acta Oto-Laryngologica. Supplement. 1993 nov;113(6):731-40.

Author

Qvortrup, K ; Rostgaard, J. / Ultrastructure of the endolymphatic duct in the rat. Fixation and preservation. I: Acta Oto-Laryngologica. Supplement. 1993 ; Bind 113, Nr. 6. s. 731-40.

Bibtex

@article{26dda52583684980b9c865a29c3909f5,
title = "Ultrastructure of the endolymphatic duct in the rat. Fixation and preservation",
abstract = "Ten rats were vascular-perfused at subphysiologic as well as physiologic pressures, 80 mmHg and 120 mmHg, respectively, employing a pressure feed-back controlled peristaltic pump and an isotonic perfusate/fixative with colloids (2{\%} Dextran) and a hypertonic perfusate/fixative without colloids, 300 and 530 mOsm, respectively. In both experiments the endolymphatic duct and sac were isolated by microdissection after primary fixation. When comparing micrographs from the two experiments we observed that rats perfused at low pressure in isotonic fixative with colloids added had non-dilated lateral intercellular spaces and a subepithelial ground substance loaded with uniformly arranged microfibrils, not previously discovered. In rats perfused at high pressure in hypertonic fixative with no colloids added, we observed an edematous state in the subepithelial space between the solid bony aqueduct and the endolymphatic duct, with a concomitant derangement of previously well organized microfibrils. Furthermore, the epithelium was quite clearly displaced from the capillaries, the intercellular spaces were widely dilated and the endolymphatic duct was compressed into its lumen. Methods of fixation and current theories of endolymph resorption by the endolymphatic duct are discussed.",
keywords = "Animals, Endolymphatic Duct, Histological Techniques, Rats, Rats, Wistar, Tissue Fixation, Journal Article, Research Support, Non-U.S. Gov't",
author = "K Qvortrup and J Rostgaard",
year = "1993",
month = "11",
language = "English",
volume = "113",
pages = "731--40",
journal = "Acta Oto-Laryngologica. Supplement",
issn = "0365-5237",
publisher = "Taylor & Francis",
number = "6",

}

RIS

TY - JOUR

T1 - Ultrastructure of the endolymphatic duct in the rat. Fixation and preservation

AU - Qvortrup, K

AU - Rostgaard, J

PY - 1993/11

Y1 - 1993/11

N2 - Ten rats were vascular-perfused at subphysiologic as well as physiologic pressures, 80 mmHg and 120 mmHg, respectively, employing a pressure feed-back controlled peristaltic pump and an isotonic perfusate/fixative with colloids (2% Dextran) and a hypertonic perfusate/fixative without colloids, 300 and 530 mOsm, respectively. In both experiments the endolymphatic duct and sac were isolated by microdissection after primary fixation. When comparing micrographs from the two experiments we observed that rats perfused at low pressure in isotonic fixative with colloids added had non-dilated lateral intercellular spaces and a subepithelial ground substance loaded with uniformly arranged microfibrils, not previously discovered. In rats perfused at high pressure in hypertonic fixative with no colloids added, we observed an edematous state in the subepithelial space between the solid bony aqueduct and the endolymphatic duct, with a concomitant derangement of previously well organized microfibrils. Furthermore, the epithelium was quite clearly displaced from the capillaries, the intercellular spaces were widely dilated and the endolymphatic duct was compressed into its lumen. Methods of fixation and current theories of endolymph resorption by the endolymphatic duct are discussed.

AB - Ten rats were vascular-perfused at subphysiologic as well as physiologic pressures, 80 mmHg and 120 mmHg, respectively, employing a pressure feed-back controlled peristaltic pump and an isotonic perfusate/fixative with colloids (2% Dextran) and a hypertonic perfusate/fixative without colloids, 300 and 530 mOsm, respectively. In both experiments the endolymphatic duct and sac were isolated by microdissection after primary fixation. When comparing micrographs from the two experiments we observed that rats perfused at low pressure in isotonic fixative with colloids added had non-dilated lateral intercellular spaces and a subepithelial ground substance loaded with uniformly arranged microfibrils, not previously discovered. In rats perfused at high pressure in hypertonic fixative with no colloids added, we observed an edematous state in the subepithelial space between the solid bony aqueduct and the endolymphatic duct, with a concomitant derangement of previously well organized microfibrils. Furthermore, the epithelium was quite clearly displaced from the capillaries, the intercellular spaces were widely dilated and the endolymphatic duct was compressed into its lumen. Methods of fixation and current theories of endolymph resorption by the endolymphatic duct are discussed.

KW - Animals

KW - Endolymphatic Duct

KW - Histological Techniques

KW - Rats

KW - Rats, Wistar

KW - Tissue Fixation

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 8291431

VL - 113

SP - 731

EP - 740

JO - Acta Oto-Laryngologica. Supplement

JF - Acta Oto-Laryngologica. Supplement

SN - 0365-5237

IS - 6

ER -

ID: 176771188