Forskning ved Københavns Universitet - Københavns Universitet

Forside

Ultrathin sectioning for electron microscopy: the distilled water in the knife trough may extract phosphatase reaction products from the sections

Publikation: Bidrag til tidsskriftTidsskriftartikel

During an electron microscopical study of the localization of the nucleoside diphosphatase IDPase in Reissner's membrane of the inner ear, it was discovered that the distilled water in the knife trough produced an annoying artefact. It dissolved all the lead phosphate reaction product from the sections, and thus converted a positive phosphatase reactivity to a false negative one. The water in the knife trough had a pH of approximately 5.4. Calculations showed that this is an expected acidity, if CO2 in the air equilibrates with distilled water, and that there is 200,000 times more acid in the trough than necessary to dissolve all the reaction product from a ribbon of ultrathin sections. Experiments showed that the artefact could be avoided by adjusting the pH to neutrality with dilute ammonia.

OriginalsprogEngelsk
TidsskriftJournal of Microscopy
Vol/bind156
Udgave nummerPt 2
Sider (fra-til)253-7
Antal sider5
ISSN0022-2720
StatusUdgivet - nov. 1989

ID: 176538686