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Uranyl photoprobing of conformational changes in DNA induced by drug binding

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The effect on DNA conformation upon binding of the bis-intercalator, N,N'-diacridinyl spermidine is studied by uranyl mediated DNA photocleavage and gel retardation. It is shown that a characteristic A-tract correlated 10 base pair modulation of the uranyl photocleavage of bent (kinetoplast) DNA is suppressed upon binding of the diacridine. Likewise, the anomalous slow gel electrophoretic migration of bent-DNA is abolished by diacridine binding. At higher diacridine/DNA ratios a DNA conformation in which G-residues become hypersensitive to uranyl photocleavage is induced.

OriginalsprogEngelsk
TidsskriftAnti-Cancer Drug Design
Vol/bind5
Udgave nummer1
Sider (fra-til)105-10
Antal sider6
ISSN0266-9536
StatusUdgivet - feb. 1990

ID: 203632167