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Uranyl salts as photochemical agents for cleavage of DNA and probing of protein-DNA contacts

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Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (lambda approximately 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the lambda-repressor/ORI operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone.

OriginalsprogEngelsk
TidsskriftFEBS letters
Vol/bind235
Udgave nummer1-2
Sider (fra-til)122-4
Antal sider3
ISSN0014-5793
StatusUdgivet - 1 aug. 1988

ID: 203631297