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yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Stefan Frank
  • Gaurav Ahuja
  • Deniz Bartsch
  • Nicole Russ
  • Wenjie Yao
  • Joseph Chao-Chung Kuo
  • Jens-Peter Derks
  • Vijay Suresh Akhade
  • Yulia Kargapolova
  • Theodore Georgomanolis
  • Marie Gramm
  • Lilija Brant
  • Rizwan Rehimi
  • Natalia Emilse Vargas
  • Alina Kuroczik
  • Tsun-Po Yang
  • Raja Ghazanfar Ali Sahito
  • Julia Franzen
  • Juergen Hescheler
  • Agapios Sachinidis
  • Martin Peifer
  • Alvaro Rada-Iglesias
  • Meena Kanduri
  • Ivan G Costa
  • Chandrasekhar Kanduri
  • Argyris Papantonis
  • Leo Kurian

Human protein-coding genes are often accompanied by divergently transcribed non-coding RNAs whose functions, especially in cell fate decisions, are poorly understood. Using an hESC-based cardiac differentiation model, we define a class of divergent lncRNAs, termed yin yang lncRNAs (yylncRNAs), that mirror the cell-type-specific expression pattern of their protein-coding counterparts. yylncRNAs are preferentially encoded from the genomic loci of key developmental cell fate regulators. Most yylncRNAs are spliced polyadenylated transcripts showing comparable expression patterns in vivo in mouse and in human embryos. Signifying their developmental function, the key mesoderm specifier BRACHYURY (T) is accompanied by yylncT, which localizes to the active T locus during mesoderm commitment. yylncT binds the de novo DNA methyltransferase DNMT3B, and its transcript is required for activation of the T locus, with yylncT depletion specifically abolishing mesodermal commitment. Collectively, we report a lncRNA-mediated regulatory layer safeguarding embryonic cell fate transitions.

OriginalsprogEngelsk
TidsskriftCell Stem Cell
Vol/bind24
Udgave nummer2
Sider (fra-til)318-327.e8
ISSN1934-5909
DOI
StatusUdgivet - 7 feb. 2019
Eksternt udgivetJa

ID: 225433404