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Structure of the class D GPCR Ste2 dimer coupled to two G proteins

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G-protein-coupled receptors (GPCRs) are divided phylogenetically into six classes(1,2), denoted A to F. More than 370 structures of vertebrate GPCRs (belonging to classes A, B, C and F) have been determined, leading to a substantial understanding of their function(3). By contrast, there are no structures of class D GPCRs, which are found exclusively in fungi where they regulate survival and reproduction. Here we determine the structure of a class D GPCR, the Saccharomyces cerevisiae pheromone receptor Ste2, in an active state coupled to the heterotrimeric G protein Gpa1-Ste4-Ste18. Ste2 was purified as a homodimer coupled to two G proteins. The dimer interface of Ste2 is formed by the N terminus, the transmembrane helices H1, H2 and H7, and the first extracellular loop ECL1. We establish a class D1 generic residue numbering system (CD1) to enable comparisons with orthologues and with other GPCR classes. The structure of Ste2 bears similarities in overall topology to class A GPCRs, but the transmembrane helix H4 is shifted by more than 20 angstrom and the G-protein-binding site is a shallow groove rather than a cleft. The structure provides a template for the design of novel drugs to target fungal GPCRs, which could be used to treat numerous intractable fungal diseases(4).

A cryo-electron microscopy structure of the yeast pheromone receptor Ste2, a class D G-protein-coupled receptor, in its active state reveals that Ste2 is a homodimer that couples to two G proteins.

Original languageEnglish
JournalNature
Volume589
Pages (from-to)148-153 +app 24 pp
ISSN0028-0836
DOIs
Publication statusPublished - 2021

    Research areas

  • ALPHA-FACTOR RECEPTOR, NUCLEOTIDE-BINDING PROTEIN, DUAL LIPID MODIFICATION, SACCHAROMYCES-CEREVISIAE, MOLECULAR SIMULATION, PHEROMONE RECEPTOR, ROLES, SOFTWARE, DYNAMICS, MODEL

ID: 254989003